Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-4448 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1448 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-931 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-930 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-799 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-4448 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-4448 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1448 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-930 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-931 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-799 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-1448 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-930 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-931 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-799 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1448 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-930 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-931 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-799 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-1448 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-930 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-931 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-799 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-1448 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-930 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-931 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-799 Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1448
Normal numbers of sporozoites are formed that are infective to cultured hepatocytes. Parasites arrest during development in hepatocytes after a period of growth. In most parasites no merozoites are formed as shown by the low level of MSP1 expression. After intravenous injection of sporozoites, 1 out of 60 mice developed a blood stage infection
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-930
Mutant sporozoites showed normal gliding motility and WT-levels of hepatocyte invasion. Mice infected with either 1 or 5x104 Pbsequestrin sporozoites, intravenously, had a 2-3 day delay in blood-stage patency when compared to WT sporozoites infections and 4 out of 11 mice did not develop a blood-stage infection after inoculation with 1x104 sporozoites. These observations show that liver stage development is strongly affected in the absence of sequestrin and the 2-3 day prolonged prepatent period is indicative of a >99% reduction in liver-stage development. Pbsequestrin liver stages have normal morphology, with respect to cell division, size and PVM formation at 24hpi. However at 48hpi, as determined by staining with anti-MSP1 antibodies, all liver-stage parasites were MSP1 negative. To investigate the maturation of these parasites, 54hpi parasites were examined using anti-MSP1 and anti-EXP1 antibodies. Over 60% WT parasites at this time point were strongly MSP1 positive, whereas the majority of Pbsequestrin parasites were MSP1 negative, with only around 7% of parasites exhibiting similar MSP1 staining.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-931
Mutant sporozoites showed normal gliding motility and WT-levels of hepatocyte invasion. Mice infected with either 1 or 5x104 Pbsequestrin sporozoites, intravenously, had a 2-3 day delay in blood-stage patency when compared to WT sporozoites infections and 4 out of 11 mice did not develop a blood-stage infection after inoculation with 1x104 sporozoites. These observations show that liver stage development is strongly affected in the absence of sequestrin and the 2-3 day prolonged prepatent period is indicative of a >99% reduction in liver-stage development. Pbsequestrin liver stages have normal morphology, with respect to cell division, size and PVM formation at 24hpi. However at 48hpi, as determined by staining with anti-MSP1 antibodies, all liver-stage parasites were MSP1 negative. To investigate the maturation of these parasites, 54hpi parasites were examined using anti-MSP1 and anti-EXP1 antibodies. Over 60% WT parasites at this time point were strongly MSP1 positive, whereas the majority of Pbsequestrin parasites were MSP1 negative, with only around 7% of parasites exhibiting similar MSP1 staining.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-799
Sporozoites show normal hepatocyte traversal and invasion Strongly reduced liver stage development. In mice a delay of the prepatent period of 1.5 days after inoculation of sporozoites. Normal livers stage development up to 24 hour after invasion of hepatocytes. Maturing liver stage schizonts show abberrant morphology and merozoite formation is impaired (see also 'Additional Information')
Imported from RMgmDB

More information

PlasmoDB PY17X_1004400
GeneDB PY17X_1004400
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) null
Orthologs PBANKA_1003000 , PCHAS_1003900 , PF3D7_0405300
Google Scholar Search for all mentions of this gene