Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-4567 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-353 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-45 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA
Possible
RMgm-4160 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-4743 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-4567 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-4567 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-353 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-45 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Asexual
No difference
RMgm-4743 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-353 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-45 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-353 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-45 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-353 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-45 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-353 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-45 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-4160
Normal sporozoite production. Sporozoites fail to establish an infection in hepatocytes. Analysis of the kinetics of Pbp36 sporozoite invasion by FACS, in comparison to control PbGFP sporozoites, revealed that genetic ablation of p36 abrogates sporozoite productive invasion of HepG2 (SR-BI-dependent entry pathway) and Hepa1-6 cells (CD81-dependent entry pathway)(see below for more phenotype information).
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-353
See remarks below ('Phenotype')
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-45
Gliding motility is similar to wild type sporozoites. Hepatocyte traversal activity in vitro (HepG2) was higher than of wild type sporozoites (~7 times higher). Hepatocyte invasion of mutant sporozoites was significantly decreased (~9% of that of wild type). Liver stage development is strongly impaired and parasites do not develop into the schizont stage. Infectivity of sporozoites as measured by infection of rats (Wistar) by intravenous inoculation of sporozoites was reduced (1000 times compared to wildtype).
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-4160
Normal sporozoite production. Sporozoites fail to establish an infection in hepatocytes. Analysis of the kinetics of Pbp36 sporozoite invasion by FACS, in comparison to control PbGFP sporozoites, revealed that genetic ablation of p36 abrogates sporozoite productive invasion of HepG2 (SR-BI-dependent entry pathway) and Hepa1-6 cells (CD81-dependent entry pathway)(see below for more phenotype information).
Imported from RMgmDB

More information