Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-936 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-934 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-933 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-932 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) Imported from PlasmoGEM
P. falciparum 3D7
Possible
25407681 Theo Sanderson, Wellcome Trust Sanger Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-936 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-936 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-936 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-936 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
No difference
RMgm-936 Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-936
Development of Pyb9 was like WT parasites in the blood (as they develop a 0.5-2% parasitemia after 8 days from an infection initiated by a single parasite) and in mosquito-stage development (normal production of oocysts and sporozoites). Liver-stage development was analysed in BALB/c mice after inoculation of 104 or 2x105 sporozoites by in vivo imaging and analysis of subsequent blood-stage infections. In vivo imaging of mice infected with 2x105 Pyb9 sporozoites showed faint luminescent signals in the liver at 3hpi that were significantly higher than background values of uninfected mice. At 40hpi none of the infected mice showed luminescence signals above background. A blood-stage infection was only detected in 1 out of 8 mice infected with 2x105 sporozoites with a long prepatent period of 10 days. These results indicate that Pyb9 parasites, like P. berghei b9, are severely compromised in liver stage development.
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-934 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-933 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-932 Imported from RMgmDB
P. falciparum 3D7 Asexual
No difference
25407681 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Gametocyte
No difference
RMgm-934 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-933 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-932 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-934 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-933 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-932 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-934 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-933 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-932 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-934 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-933 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-932 Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-934
Normal sporozoite production. Sporozoites showed normal gliding motility and WT-levels of hepatocyte invasion. When Swiss or BALB/c mice were infected by intravenous inoculation of either 1 or 5x104 Pbb9 sporozoites none of the mice developed blood-stage infections. When C57BL6 mice were infected with a high dose of 5x104 Pbb9 sporozoites, 10-20% developed a blood-stage infection with a 3-4 days prolonged prepatent period. Immunofluorescence analyses show that Pbb9 parasites arrest early after invasion of hepatocytes. Pbb9 sporozoites exhibit normal hepatocyte invasion, but at 24hpi most intra-cellular parasites had disappeared and only a few small parasites could be observed with a size that was similar to 5-10 hpi liver stages. Analysis of Pbb9 parasites in the liver, using real-time in vivo imaging, confirmed the early growth-arrest observed in cultured hepatocytes.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-933
Normal sporozoite production. Sporozoites showed normal gliding motility and WT-levels of hepatocyte invasion. When Swiss or BALB/c mice were infected by intravenous inoculation of either 1 or 5x104 Pbb9 sporozoites none of the mice developed blood-stage infections. When C57BL6 mice were infected with a high dose of 5x104 Pbb9 sporozoites, 10-20% developed a blood-stage infection with a 3-4 days prolonged prepatent period. Immunofluorescence analyses show that Pbb9 parasites arrest early after invasion of hepatocytes. Pbb9 sporozoites exhibit normal hepatocyte invasion, but at 24hpi most intra-cellular parasites had disappeared and only a few small parasites could be observed with a size that was similar to 5-10 hpi liver stages. Analysis of Pbb9 parasites in the liver, using real-time in vivo imaging, confirmed the early growth-arrest observed in cultured hepatocytes.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-932
Normal sporozoite production. Sporozoites showed normal gliding motility and WT-levels of hepatocyte invasion. When Swiss or BALB/c mice were infected by intravenous inoculation of either 1 or 5x104 Pbb9 sporozoites none of the mice developed blood-stage infections. When C57BL6 mice were infected with a high dose of 5x104 Pbb9 sporozoites, 10-20% developed a blood-stage infection with a 3-4 days prolonged prepatent period. Immunofluorescence analyses show that Pbb9 parasites arrest early after invasion of hepatocytes. Pbb9 sporozoites exhibit normal hepatocyte invasion, but at 24hpi most intra-cellular parasites had disappeared and only a few small parasites could be observed with a size that was similar to 5-10 hpi liver stages. Analysis of Pbb9 parasites in the liver, using real-time in vivo imaging, confirmed the early growth-arrest observed in cultured hepatocytes.
Imported from RMgmDB

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