Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA
Refractory
RMgm-4445 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Localization of Pfstomatin to the apical organelles of merozoites and association of parasite rhoptry protein RhopH2 . Indirect immunofluorescence assays of: (i–iii) free merozoites probed with antibodies to Pfstomatin (i; green), and Mito Tracker (ii; red); the relative lack of co-localization of these markers is shown in the merged panel iii. iv–vi intracellular schizont-stage parasites expressing GFP in the apicoplast were probed with antibodies to Pfstomatin (iv; red), GFP (v; green); the relative lack of co-localization of these markers is shown in the merged panel vi. vii–ix free merozoites probed with antibodies to: Pfstomatin (vii; green), RhopH2 (viii; red); the extent of co-localization is shown in the merged panel ix. All images shown are single optical sections. The nucleus (blue) was stained with Hoechst 33342. Scale bars correspond to 2 mm.Hiller NL, Akompong T, Morrow JS, Holder AA, Haldar K. Identification of a stomatin orthologue in vacuoles induced in human erythrocytes by malaria parasites. A role for microbial raft proteins in apicomplexan vacuole biogenesis. J Biol Chem. 2003 278:48413-21.

See original on MMP

Localization of Pfstomatin and RhopH2 to the PVM in ring-infected erythrocytes (0–8 h post-invasion). Cells were subjected to a standard indirect immunofluorescence assay where samples were permeabilized with saponin and probed with antibodies to: Pfstomatin (Ai; green), RhopH2 (Bi; green), and the PVM marker EXP-1 (Aii and Bii; red); the merged images are shown in Aiii and Biii. In C and D, ring-infected erythrocytes were treated with tetanolysin and incubated with antibodies to: Pfstomatin (Ci; red), RhopH2 (Di; red) and the PPM marker MSP-1 (Cii and Dii; green); the merged images are shown in Ciii and Diii. In E and F, rings treated with tetanolysin and permeabilized with saponin were incubated with antibodies to: Pfstomatin (Ei; red), RhopH2 (Fi; red), and MSP-1 (Eii and Fii; green); the merged images are shown in Diii and Fiii. All images shown are single optical section. The nucleus (blue) was stained with Hoechst 33342. Scale bars represent 2 mm. Hiller NL, Akompong T, Morrow JS, Holder AA, Haldar K. Identification of a stomatin orthologue in vacuoles induced in human erythrocytes by malaria parasites. A role for microbial raft proteins in apicomplexan vacuole biogenesis. J Biol Chem. 2003 278:48413-21.

See original on MMP

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