Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-379 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-377 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
25387830 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-379 Imported from RMgmDB
P. yoelii yoelii 17X Asexual
No difference
RMgm-377 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-379 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-377 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-379 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-377 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-379 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-377 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
No difference
RMgm-379 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
No difference
RMgm-377 Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-379
BALB/c mice intravenously injected with 1x10(4)-5x10(4) salivary gland sporozoites did not develop blood stage infections. Analysis of in vitro cultured liver stages showed significant defects in terms of growth and nuclear division. At 24 h pi, no difference was observed between mutant and wild type liver stages with regard to protein expression and parasite size. During late liver stage development (43 and 52 h pi), no expression was detected of the merozoite-specific protein MSPI in mutant parasites. Mutant parasites showed significant defects in terms of growth and nuclear division and the average size of the mutant parasites at 43 h pi was approximately 30% of wild type parasites.
Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-377
BALB/c mice intravenously injected with 1x10(4)-5x10(4) salivary gland sporozoites did not develop blood stage infections. Analysis of in vitro cultured liver stages showed significant defects in terms of growth and nuclear division. At 24 h pi, no difference was observed between mutant and wild type liver stages with regard to protein expression and parasite size. During late liver stage development (43 and 52 h pi), no expression was detected of the merozoite-specific protein MSPI in mutant parasites. Mutant parasites showed significant defects in terms of growth and nuclear division and the average size of the mutant parasites at 43 h pi was approximately 30% of wild type parasites.
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7 Asexual
No difference
25387830 Theo Sanderson, Wellcome Trust Sanger Institute

Imaging data (from Malaria Metabolic Pathways)

Immunofluorescent analyses were performed on P. falciparum 3D7 using anti-LplA2 and anti-aLipDH (aE3) antibodies (specifically staining the apicoplast of the parasites). Panel 1 shows staining of an organelle distinct from the apicoplast, probably being the mitochondrion. Panel 2 shows costaining of both antibodies, suggesting LplA2 is located in the apicoplast, and panel 3 shows staining of both the apicoplast and potentially the mitochondrion. Scale bars: 5 mm.Günther S, Wallace L, Patzewitz EM, McMillan PJ, Storm J, Wrenger C, Bissett R, Smith TK, Müller S. Apicoplast lipoic acid protein ligase B is not essential for Plasmodium falciparum. PLoS Pathog. 2007 Dec;3(12):e189.

See original on MMP

Localization of apicoplast and mitochondrial LipDH. The localization of aLipDH and mLipDH was investigated by expressing the putative N-terminal presequences fused to GFP in P. falciparum intraerythrocytic parasites. A and B. Colocalization with antibodies directed against the apicoplast-resident acyl-carrier protein PFB0385w (ACP; A) and with MitoTracker Red (B) demonstrates that the putative apicoplast targeting presequence of aLipDH targets the marker protein GFP exclusively to the apicoplast. C–E. Colocalization experiments with the apicoplast protein ACP (C) and with the two mitochondrial markers MitoTracker Red and HSP60 PF10_0153 (D and E) indicate that mLipDH is localized exclusively in the mitochondrion.McMillan PJ, Stimmler LM, Foth BJ, McFadden GI, Müller S. The human malaria parasite Plasmodium falciparum possesses two distinct dihydrolipoamide dehydrogenases. Mol Microbiol. 2005 55:27-38. Copyright John Wiley & Sons Ltd. 2010.

See original on MMP

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