Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-5050 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-123 Imported from RMgmDB
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Ookinete
Difference from wild-type
RMgm-5050
Ookinetes are formed but no traversal through mosquito midgut cells
Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
Difference from wild-type
RMgm-5050
Ookinetes are formed but no traversal through mosquito midgut cells
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-123 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-123 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-123
Normal numbers of ookinetes are produced. Most (>99%) of the ookinetes do not develop into oocysts. Ookinetes also failed to infect mosquitoes when ookinetes were cultured in vitro and fed to mosquitoes via membrane feeding.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-123
No or very few oocysts are formed. Most (>99%) of the ookinetes do not develop into oocysts. However, the block in infection was not absolute, since a single oocyst was observed in two of 50 dissected mosquitoes in one experiment. Moreover, in another experiment a single sporozoite was observed in salivary gland dissections.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-123
No or very few oocysts are formed. Most (>99%) of the ookinetes do not develop into oocysts. However, the block in infection was not absolute, since a single oocyst was observed in two of 50 dissected mosquitoes in one experiment. Moreover, in another experiment a single sporozoite was observed in salivary gland dissections.Despite the low numbers of sporozoites, infected mosquitoes were able to transmit parasites to C57BL/6 mice.
Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-123 Imported from RMgmDB

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