Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-171 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-38 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-35 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) Imported from PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-171 Imported from RMgmDB
P. yoelii yoelii 17X Asexual
No difference
RMgm-38 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-171 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-38 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-171 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-38 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-171 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-38 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
No difference
RMgm-38 Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-38
In vitro invasion of hepatocytes by the sporozoites is not affected. Liver stage development is strongly impaired. In vivo (BALB/c mice), the number of liver stages at 2 hours after intravenous injection of sporozoites is reduced by 60%. The number of liver stages at 24h were reduced by >95%. liver stages could not be detected at 40h after infection. Liver stages were morphologically similar to wt liver stages at 6 and 12h after infection, a time when the intrahepatocytic transformation from elongate sporozoite to spherical trophozoites commences. Antibody staining for HEP17, a parasitophorous vacuole membrane (PVM)protein, showed a typical circumferential pattern, which indicates that the PVM is present. Liver stages did not develop into schizonts but appeared to be arrested at the trophozoite stage. Arrested liver stages did not persist in the liver after 40h. Infection of BALB/c mice by intravenous inoculation of high numbers of sporozoites (50.000) did not result in blood stage infection.
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-35 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) Imported from PlasmoGEM
P. berghei ANKA Gametocyte
No difference
RMgm-35 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-35 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-35 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-35 Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-35
In vitro invasion of hepatocytes by the sporozoites is not affected. Liver stage development is strongly impaired. Liver stage numbers are reduced by ~50% twenty four hours after invasion of HepG2 cells. At later time points, mature liver schizonts are absent. Only developmentally arrested liver forms persist.Infection of mice (C57BL/6) by bite of infected mosquitoes or subcutaneous inoculation of sporozoites did not result in blood stage infection. Only intraveneous inoculation of very high numbers of sporozoites (50.000) resulted in blood stage infection in ~50% of the mice. These mice showed a prolonged prepatent period.
Imported from RMgmDB

More information

PlasmoDB PY17X_0502200
GeneDB PY17X_0502200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) null
Orthologs PBANKA_0501200 , PCHAS_0501300
Google Scholar Search for all mentions of this gene