Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-148 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-148 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-148 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-148 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-148 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-148
Oocysts appeared to develop normally and formed large numbers of sporozoites. However, closer examination of these sporozoites revealed that they were of abnormal shape and some 2030% smaller in size. Each sporozoite possessed a single enlarged, protruding area associated with the position of the nucleus. The position of these protrusions varied between sporozoites from being located at the posterior end to being positioned near the middle of the sporozoite.The mutant sporozoites accumulated in the hemolymph and did not invade the salivary glands. Gliding motility was strongly reduced. Mutant sporozoites were able to attach normally to the glass surface and undergo circular gliding, but their gliding speed was fivefold slower than that of wild type sporozoites.Inoculation of purified oocyst-derived/hemolymph sporozoites into mice did not result in infection of the mice.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-148
Inoculation of purified oocyst-derived/hemolymph sporozoites into mice did not result in infection of the mice.
Imported from RMgmDB

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