Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
RMgm-4381 Imported from RMgmDB
P. berghei ANKA
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
20195509 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

PfSIP2-N localizes to P. falciparum chromosome end clusters. (A) IFA detects discrete perinuclear PfSIP2-N-HA signals in late trophozoites (LT) and early (ES) and late (LS) schizont stage 3D7/SIP2-N-HA parasites. The expression cassette is schematically depicted on the left. (B) Co-localisation of PfSIP2-N with PfHP1-containing subtelomeric heterochromatin in late trophozoites (LT) and early (ES) and late (LS) schizonts in the double transgenic parasite line 3D7/SIP2-N-HA/HP1-Ty (over-expressing both proteins as epitope-tagged versions simultaneously). Expression cassettes are schematically depicted on the left. Indirect immunofluorescence (IFA) microscopy identified discrete PfSIP2-N-HA foci at the nuclear periphery with increasing numbers of foci in replicating stages (A) consistent with chromosome end clusters. PfHP1 is a heterochromatic marker. Both proteins co-localized at the nuclear periphery (B). Flueck C, Bartfai R, Niederwieser I, Witmer K, Alako BT, Moes S, Bozdech Z, Jenoe P, Stunnenberg HG, Voss TS. A major role for the Plasmodium falciparum ApiAP2 protein PfSIP2 in chromosome end biology. PLoS Pathog. 2010 6:e1000784.

See original on MMP

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