Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-4104 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-207 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. yoelii yoelii 17X
Possible
RMgm-4388 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-4104 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-207 Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. yoelii yoelii 17X Asexual
No difference
RMgm-4388 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-4104 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-207 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-4388 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-4104
Normal gametocyte production and gamete formation. Evidence for normal fertilisation. Formation of retort shaped, immature ookinetes but no mature ookinete production.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-207
The mutant parasites formed morphologically normal female and male gametocytes and exhibited normal exflagellation rates. However, they lacked the ability to infect mosquitoes as no oocysts or sporozoites were found in the mosquito. In culture, mutant parasites formed zygotes at normal conversion rates and differentiated into retort forms by 9 h after fertilization, as in wild type parasites. Subtle morphological differences between wild type and mutant parasites became visible 12 h after fertilization with a swelling protrusion in the retort forms. Mature ookinetes showed an aberrant morphology with an pear-shaped form, different from the banana (sword)-like form of wild type ookinetes.
Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
Difference from wild-type
RMgm-4388
Normal numbers of gametocytes are produced. Only ookinetes with aberrant morphology are produced.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4104
No mature ookinete production. No oocyst production.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-207
No oocyst and sporozoite production
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-207
No oocyst and sporozoite production
Imported from RMgmDB

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