Last updated 1 week ago

Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA
Possible
RMgm-4881 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-4765 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Asexual
No difference
RMgm-4881 Imported from RMgmDB
P. yoelii yoelii 17X Asexual
No difference
RMgm-4765 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-4881 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
Difference from wild-type
RMgm-4765
normal production of gametocytes; normal exflagellation
Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-4881 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4881
Normal numbers of oocysts. However, at day 17 only 2% of them had entered sporulation, as compared to 50% of WT oocysts. This striking defect correlated with prominent swelling of the FT2-deficient oocysts.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-4881
Extremely low numbers of ft2sporozoites (~1% of WT) associated with mosquito salivary glands. Sporozoites are not infectious to mice and in vitro hepatocyte cultures.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-4881
The few sporozoites formed are not infectious to mice and in vitro hepatocyte cultures.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Cytolocalization of P. falciparum folate transporters PfFT1 and PfFT2. A, fluorescence signals given by P. falciparum transformed with a PfFT1-GFP C-terminal fusion construct are shown. A trophozoite (top panels) shows a signal that coincides with the parasites plasma membrane. Dividing stages of early and late schizonts are in the two lower panels. In the schizonts the labeling of merozoite plasma membranes is apparent. B, indirect immunofluorescence of PfFT2 is shown. Permeabilized cells were labeled with an anti-PfFT2 antibody. Again trophozoites (upper and middle panels) as well as merozoites in the schizont in the lower panel presented strong plasma membrane signal. Bars represent 2mm.Salcedo-Sora JE, Ochong E, Beveridge S, Johnson D, Nzila A, Biagini GA, Stocks PA, O'Neill PM, Krishna S, Bray PG, Ward SA. The molecular basis of folate salvage in Plasmodium falciparum: characterization of two folate transporters. J Biol Chem. 2011286(52):44659-68.

See original on MMP

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