Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-5209 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
31559936
"PfDPY19 gene disruption was not associated with a growth phenotype, not even under endoplasmic reticulum (ER)-stressing conditions that could impair protein folding. The data presented in this work strongly suggests that PfDPY19 is unlikely to play a critical role in the asexual blood stages of the parasite, at least under in vitro conditions. "
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7
Possible
35906227
\'We further show that genetic disruption of DPY19 in P. falciparum is necessary and sufficient to prevent C-mannosylation in the parasite, impair egress following gametocyte activation and abrogate ookinete infection of the mosquito midgut. Complementation of DPY19 restored the transmission defect and re-instated C-mannosylation activity, definitively validating tryptophan C-mannosylation as essential for P. falciparum infection of the mosquito, which is critical for onward transmission of the parasite.\'
Theo Sanderson, Francis Crick Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-5209 Imported from RMgmDB
P. falciparum 3D7 Asexual
No difference
31559936
"PfDPY19 gene disruption was not associated with a growth phenotype, not even under endoplasmic reticulum (ER)-stressing conditions that could impair protein folding. The data presented in this work strongly suggests that PfDPY19 is unlikely to play a critical role in the asexual blood stages of the parasite, at least under in vitro conditions. "
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Gametocyte
No difference
RMgm-5209 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-5209
Normal, wild-type gametocyte and ookinete production. However, ookinetes showed strongly reduced motility and motion velocity. No oocysts are formed
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-5209
No oocyst formation
Imported from RMgmDB
P. falciparum 3D7 Oocyst
Attenuated
35906227
\'We further show that genetic disruption of DPY19 in P. falciparum is necessary and sufficient to prevent C-mannosylation in the parasite, impair egress following gametocyte activation and abrogate ookinete infection of the mosquito midgut. Complementation of DPY19 restored the transmission defect and re-instated C-mannosylation activity, definitively validating tryptophan C-mannosylation as essential for P. falciparum infection of the mosquito, which is critical for onward transmission of the parasite.\'
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-5209
No oocyst and sporozoite formation
Imported from RMgmDB

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