Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-142 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-142 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
No difference
RMgm-142 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-142
Mutants developed gametocytes and formed ookinetes in vitro in similar numbers to, and morphologically indistinguishable from, wild type parasites in Giemsa-stained preparations. Reduced numbers of oocysts (reduction of 60-85%) are produced in A. stephensi mosquitoes.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-142
Normal numbers of ookinetes are produced. Reduced numbers of oocysts (reduction of 60-85%) are produced in A. stephensi mosquitoes. Although oocyst numbers were markedly reduced in mutant-infected mosquitoes, those oocysts that developed were morphologically indistinguishable from wild type oocysts of comparable age, and formed large numbers of sporozoites similar to wild type oocysts.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-142
Mutant sporozoites appeared morphologically normal and successfully invaded the salivary glands. Mosquitoes that were infected with mutant sporozoites successfully transmitted the parasite to mice.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-142
Mutant sporozoites appeared morphologically normal and successfully invaded the salivary glands. Mosquitoes that were infected with mutant sporozoites successfully transmitted the parasite to mice.
Imported from RMgmDB

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