Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-4876 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-4876 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-4876 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-4876 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4876
There were significantly fewer oocysts in mre11 lines, and the vast majority of these oocysts were significantly smaller than WT-GFP oocysts. In none of the mre11 oocysts was there any evidence of sporozoite development, and those of similar size to WT-GFP oocysts had patterns of fragmented GFP expression and reduced Hoechst DNA-staining. Investigation of the sex-cell lineage of this defect revealed that it could be rescued by crossing mre11 parasites with map2 (male-defective) cells, suggesting that the function of MRE11 is inherited through the female.The mre11 oocysts had a fully formed wall but the cytoplasm exhibited evidence of degeneration, with vacuolization and organelle breakdown, while the nuclei showed marked swelling of the nuclear membranes
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-4876
no sporozoite formation
Imported from RMgmDB

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