|P. berghei ANKA||Asexual||
|P. falciparum 3D7||Asexual||
| 32019597 (Knock down)
The parasitemia of PfSWIB∆ showed a significant decline at 96 h during in vitro culture compared with the PfSWIB and 3D7 lines (P < 0.0001). qPCR and RNA-seq analysis confirmed that depletion of PfSWIB not only silences upsA, upsC and partial upsB var genes, as well as removes the silencing of partial upsB var genes at the ring stage in PfSWIB∆ line, but also leads to aberrant expression of upsA and partial upsB/upsC var genes at the mature stage of P. falciparum, during a single 48-h life-cycle.
|Theo Sanderson, Francis Crick Institute|
Live cellular localization of GFP-tagged PfSWIB within Plasmodium falciparum. A distinct cytoplasmic GFP signal was observed in all life stages under normal conditions, whereas exposure to 41 °C for 2 h resulted in nuclear localization, 30 min after termination of heat stress. This redistributionwas short lived since a nuclear signal was absent 2–24 h afterwards. R ring stage, T trophozoite stage, S schizont stage. Scale bar in bright fieldrepresents 2.5 μmVieira WA, Coetzer TL. Localization and interactions of Plasmodium falciparum SWIB/MDM2 homologues. Malar J. 2016 Jan 20;15(1):32.See original on MMP