Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-198 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-198 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-198 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-198 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-198
Normal numbers of oocysts are produced. Normal numbers of oocyst-derived (hemolymph) sporozoites are formed. Sporozoites do not invade the salivary gland.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-198
Normal numbers of oocysts are produced. Normal numbers of oocyst-derived (hemolymph) sporozoites are formed. Sporozoites do not invade the salivary gland. Sporozoites do not transmit to the mouse by infected mosquito bite. Intravenous inoculation of sporozoites results in blood stage infections, although at a lower efficiency compared to wild type sporozoites.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-198
Sporozoites do not invade the salivary gland. Sporozoites do not transmit to the mouse by infected mosquito bite. Intravenous inoculation of sporozoites results in blood stage infections, although at a lower efficiency compared to wild type sporozoites.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

PfCRMP1 and 2 are expressed in blood-stage parasites and colocalize with PfEMP1. A. Merged images of the fluorescence patterns of antisera detecting PCRMP1 (red) and the PVM antigen, EXP1C (PF11_0224), or PfEMP1-ATS (green) as indicated show that PCRMP1 is exported to structures within P. falciparum A4-, 3D7- and T9-96-IE cytoplasm and colocalizes with PfEMP1 in the MCs of IEs. Nuclei are stained with DAPI (blue). PfCRMP1 and PfEMP1 colocalize in abnormal aggregates in the cytoplasm of T9/96 mature trophozoite-IEs. B. PfCRMP1, PfCRMP2 and PfEMP1 are detected in an indistinguishable, diffuse, punctate pattern at the surface of P. falciparum A4 schizonts (1) and in the MCs of young trophozoite-IEs (2) but are not associated with released merozoites (3). Merged and individual images of the florescence pattern of parasites stained with the anti-peptide antibodies (red) indicated on the LHS and antiserum detecting the rhoptry protein RAP1 (PFE0080c; green). r, rings; yt, young trophozoites; t, trophozoite; mt, mature trophozoites; s, schizonts. Scale bar = 5 mm.Thompson J, Fernandez-Reyes D, Sharling L, Moore SG, Eling WM, Kyes SA, Newbold CI, Kafatos FC, Janse CJ, Waters AP. Plasmodium cysteine repeat modular proteins 1-4: complex proteins with roles throughout the malaria parasite life cycle. Cell Microbiol. 2007 9:1466-80. Copyright John Wiley & Sons Ltd. 2010.

See original on MMP

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