Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

The SP-GFP-BirA* construct is located in the PV and is able to biotinylate proteins in this compartment. Immune fluorescence assay (IFA) of SP-GFP-BirA* expressing parasites grown in the presence of biotin show co-localisation of biotinylated protein (detected using streptavidin) with GFPBirA* (upper panel) and ETRAMP5 (lower panel). To test whether BirA* can biotinylate proteins in the PV we grew the SP-GFP-BirA* expressing parasites in the presence of biotin. Immuno and streptavidin-fluorescence assays showed that this resulted in biotinylation of proteins in the parasite periphery and that the detected biotin co-localized with the SP-GFP-BirA* construct and with the PVM marker ETRAMP5.Khosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T. Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID. Int J Med Microbiol. 2017 Jul 27. [Epub ahead of print]

See original on MMP

More information

PlasmoDB PVP01_0504300
GeneDB PVP01_0504300
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) null
Orthologs PF3D7_0830400 , PKNH_1324200 , PVX_088855
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