Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Localization of PfeIF2b. Immunolocalization assays.Asynchronous cultures of PfeIF2b-Hatag recombinant strain of P.falciparum 3D7were fixed with formalin and paraffin embedded. Sections were incubated with an anti-HAtag(biotin) antibody recognized by a streptavidine-Alexafluor488-labeled conjugated added with DAPI to label nuclei. Fluorescence staining was analyzed using a Zeiss LSM880 confocal microscope.The merged image of the doubl estained (PfeIF2b-HAtag,DAPI) and differential interference contrast (DIC )images are also presented. Immunofluorescence assays revealed a cytoplasmic localization of PfeIF2b in the trophozoite (panel1), young schizont (panel2), or mature schizont (panel3) stages. Note that no staining was observed in ring-stage. No staining was observed when the primary antibody (anti-HA) was omitted (not shown). Bar = 5 μm.Tellier G, Lenne A, Cailliau-Maggio K, Cabezas-Cruz A, Valdés JJ, Martoriati A, Aliouat el M, Gosset P, Delaire B, Fréville A, Pierrot C, Khalife J. Identification of Plasmodium falciparum Translation Initiation eIF2β Subunit: Direct Interaction with Protein Phosphatase Type 1. Front Microbiol. 2016 26;7:777.

See original on MMP

More information

PlasmoDB PKNH_1470600
GeneDB PKNH_1470600
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK12_4930c, PKH_146830
Orthologs PBANKA_1463500 , PCHAS_1465800 , PF3D7_1250600 , PVP01_1467700 , PVX_101425 , PY17X_1466100
Google Scholar Search for all mentions of this gene