Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
33124706 (Knock down)
By conditionally depleting PfMCMBP, we disrupt nuclear morphology and parasite proliferation without causing a block in DNA replication. By immunofluorescence microscopy, we show that PfMCMBP depletion promotes the formation of mitotic spindle microtubules with extensions to more than one DNA focus and abnormal centrin distribution. Strikingly, PfMCMBP‐deficient parasites complete cytokinesis and form aneuploid merozoites with variable cellular and nuclear sizes. Our study demonstrates that the parasite lacks a robust checkpoint response to prevent cytokinesis following aberrant karyokinesis.
Theo Sanderson, Francis Crick Institute

More information

PlasmoDB PKNH_1346200
GeneDB PKNH_1346200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK14_0495w, PKH_133550
Orthologs PBANKA_1030500 , PCHAS_1031300 , PF3D7_1412100 , PVP01_1336800 , PVX_085850 , PY17X_1032900
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