Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-1389 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1324 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA
Possible
RMgm-4835 Imported from RMgmDB
P. falciparum 3D7
Possible
23129054 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-1389 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1324 Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Asexual
No difference
RMgm-4835 Imported from RMgmDB
P. falciparum 3D7 Asexual
No difference
23129054 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Gametocyte
No difference
RMgm-1389 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-1324 Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-4835
Evidence is presented for increased gametocyte conversion rates
Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-1324 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-1324 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-1389 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-1324 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-1389 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-1324 Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Confocal microscopy of plasmodium stages immuno-stained with PfABCG antiserum. Immuno-histochemical staining of 3D7 cultured parasites with PfABCG antiserum. Columns 1-3 show PfABCG immuno-histochemical staining resolved with Alexa-fluor 488-labeled goat anti-rabbit secondary antibody (Green), counterstaining of nuclear DNA with DAPI (Blue), and differential interference contrast of infected erythrocyte, respectively. Rows A-D show early trophozoites, late trophozoites schizonts and female gametocyte stage V stained with PfABCG antiserum, respectively. Column 4 shows the merge of column 1 and 2, without Differential Interference Contrast (DIC). White arrowheads indicate the intensely stained structures beneath the parasite's plasma membrane.Edaye S, Georges E. Characterization of native PfABCG protein in Plasmodium falciparum. Biochem Pharmacol. 2015 Jul 31. [Epub ahead of print]

See original on MMP

Co-localization of PfExp-1 and PfABCG in P. falciparum-infected erythrocytes. Double immuno-histochemical staining of infected erythrocytes at trophozoites stage with antibodies against PfABCG and PfExp-1 (Panel A) and PfCRT and PfExp-1 (Panel B). Column 1 shows the nuclear DNA staining with DAPI (blue). In columns 2 and 3, PfABCG signal (green) (A) and PfCRT signal (green) (B) are resolved with Alexa-fluor 488-labeled goat anti-rabbit secondary antibody, and PfExp-1 signal (red) (A and B) is resolved with Alexa-fluor 594-labeled goat anti-rabbit secondary antibody. Columns 4 and 5 show respectively the differential interference contrast (DIC) of infected erythrocyte and the merger of column 1, 2 and 3 without DIC. The digestive vacuole membrane staining of PfCRT does not overlap with PfExp-1 staining while the plasma membrane staining of PfABCG partially overlaps with the parasitophorous vacuole membrane signal of PfExp-1 as indicated by the arrow.Edaye S, Georges E. Characterization of native PfABCG protein in Plasmodium falciparum. Biochem Pharmacol. 2015 Jul 31. [Epub ahead of print] PMID:

See original on MMP

More information

PlasmoDB PKNH_1332200
GeneDB PKNH_1332200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK14_1100c, PKH_132390
Orthologs PBANKA_1018100 , PCHAS_1018900 , PF3D7_1426500 , PVP01_1322800 , PVX_085205
Google Scholar Search for all mentions of this gene