Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
Presented at MPM 2015 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA
Refractory
RMgm-1131 Imported from RMgmDB
P. berghei ANKA
Refractory
RMgm-824 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Enhanced virulence
Presented at MPM 2015 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Upper panel: The localization of PfHly III was investigated by integrating the PfHly III-GFP fusion protein into the Dd2attb genome. The integration was done by transfecting the parasite with pLN-PfHly III and pINT. PCR analysis on transfected parasites using primers (GFP for, Hly III for and GFP rev) demonstrated the presence of pLN-PfHly III-GFP DNA. IFA with anti-His ab conjugated with alexa 488 showing Hly III coating the erythrocyte membrane. Lower panel: PfHly III-GFP localizes to the digestive vacuole of P. falciparum. Live fluorescence microscopy shows co-localization of 611 PfHly III-GFP with hemozoin. Scale bar, 7μm.Moonah S, Sanders NG, Persichetti J, Sullivan DJ Jr. Erythrocyte lysis and Xenopus laevis oocyte rupture by recombinant Plasmodium falciparum hemolysin III. Eukaryot Cell. 2014 Aug 22. [Epub ahead of print]

See original on MMP

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