Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Parasites on coverslips were fixed with paraformaldehyde and permeabilized with 0.05% saponin. The cells were probed with PfMCM2 and PfMCM6 antibodies followed by Alexafluor 555 labeled antimouse (green, PfMCM2) and Alexafluor 647 labeled anti-rabbit (red, PfMCM6) secondary antibodies. DNA was stained with DAPI (blue). Images were analyzed by DeltaVision deconvolution microscopy. The bar indicates 5mM. PfMCM2, 6, and 7 appear localized in both cytosolic and nucleosolic fractions during all intraerythrocytic stages of P. falciparum development, with increased nuclear localization in schizonts. Patterson S, Robert C, Whittle C, Chakrabarti R, Doerig C, Chakrabarti D. Pre-replication complex organization in the atypical DNA replication cycle of Plasmodium falciparum: characterization of the mini-chromosome maintenance (MCM) complex formation. Mol Biochem Parasitol. 2006 145:50-9. Copyright Elsevier 2009.

See original on MMP

More information

PlasmoDB PKNH_1116800
GeneDB PKNH_1116800
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK13_5470w, PKH_111620
Orthologs PBANKA_1131600 , PCHAS_1131100 , PF3D7_1355100 , PVP01_1116800 , PVX_114735 , PY17X_1133000
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