Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Immuno-localization of GFP chimeras in transgenic parasites using a new Plasmodium falciparum cell fixation protocol. IspE(l)-GFP and PDF(f)-GFP were fixed and incubated with anti-ACP rabbit anti-serum. IspE(l)-GFP (A and B) co-localizes with anti-ACP. PDF(f)-GFP also co-localizes with anti-ACP (C and D), although it sometimes appears to localize within a sub-region of the ACP labelling (D). Stable CS(l)-GFP expressing cells (E and F) were incubated in the presence of anti-Hsp60 from E.coli. Overlay of GFP with Hsp60 suggests this Hsp60 PF10_0153 antibody is a useful mitochondrial marker. Transmission images were captured in both phase contrast and differential interference contrast (DIC) to highlight the integrity of cells fixed under these new conditions.Tonkin CJ, van Dooren GG, Spurck TP, Struck NS, Good RT, Handman E, Cowman AF, McFadden GI. Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method. Mol Biochem Parasitol. 2004 137:13-21. Copyright Elsevier 2009.

See original on MMP

More information

PlasmoDB PKNH_1030800
GeneDB PKNH_1030800
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK5_0160c, PKH_103040
Orthologs PBANKA_1102800 , PCHAS_1102500 , PF3D7_0503100 , PVP01_1031900 , PVX_097660 , PY17X_1103900
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