Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
RMgm-562 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
22127061 Theo Sanderson, Wellcome Trust Sanger Institute

Mutant phenotypes [+]

None reported yet. Please press the '+' button above to add one.

Imaging data (from Malaria Metabolic Pathways)

Subcellular localization of PfCLK-3 and PfCLK-4 in the blood stage. Mixed asexual blood stage cultures containing trophozoites (TZ) and schizonts (SZ) cultures were fixed with methanol and prepared for IFA, using rat antisera against PfCLK-3 and mouse antisera against PfCLK-4 (green). The parasite nuclei were highlighted by Hoechst staining (blue). The asexual blood stages were labelled with rabbit antisera against PfMSP-1. Bar, 5 mm. PfCLK-3 and PfCLK-4 are mainly present in the nucleus of trophozoites, while in schizonts and gametocytes both kinases are primarily located in the cytoplasm. Particularly for PfCLK-3 a rim-associated labelling pattern was observed in the latter stages. In the IFAs, the asexual blood stage parasites were highlighted by labelling of plasmalemma-associated proteins, i.e. PfMSP-1.Kern S, Agarwal S, Huber K, Gehring AP, Strödke B, Wirth CC, Brügl T, Abodo LO, Dandekar T, Doerig C, Fischer R, Tobin AB, Alam MM, Bracher F, Pradel G. Inhibition of the SR Protein-Phosphorylating CLK Kinases of Plasmodium falciparum Impairs Blood Stage Replication and Malaria Transmission. PLoS One. 2014 Sep 4;9(9):e105732.

See original on MMP

Co-localization and control IFAs. A. Immunolabelling of PfCLK-1 with rabbit antisera (red) detected the kinase in the schizonts´ cytoplasm as well as in the nuclei, where it co-localizes with the three SR proteins (green). Schizonts were counterlabelled with rabbit antibodies against PfMSP-1 (red). All nuclei were highlighted by Hoechst staining (blue). Bar, 5 μm. The co-localization experiments confirmed that the SR proteins are solely present in the nuclei, while in the early schizont stage, PfCLK-1 is present both in the parasite nuclei and the cytoplasm. Co-localization of PfCLK-1 with the three SR proteins can be detected in distinct nuclear regions. Kern S, Agarwal S, Huber K, Gehring AP, Strödke B, Wirth CC, Brügl T, Abodo LO, Dandekar T, Doerig C, Fischer R, Tobin AB, Alam MM, Bracher F, Pradel G. Inhibition of the SR Protein-Phosphorylating CLK Kinases of Plasmodium falciparum Impairs Blood Stage Replication and Malaria Transmission. PLoS One. 2014 Sep 4;9(9):e105732.

See original on MMP

More information

PlasmoDB PKNH_0912200
GeneDB PKNH_0912200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK11_1150w, PKH_091180
Orthologs PBANKA_0933000 , PCHAS_0911300 , PF3D7_1114700 , PVP01_0915400 , PVX_091375 , PY17X_0935000
Google Scholar Search for all mentions of this gene