Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-1417 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-204 Imported from RMgmDB
P. falciparum 3D7
Refractory
26060916 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Difference from wild-type
RMgm-1417
Reduced growth of asexual blood stages in mice
Imported from RMgmDB
P. berghei ANKA Asexual
Difference from wild-type
RMgm-204
Blood stages show a small decrease in the growth rate in mice.
Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-204 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-204
Normal gametocyte production. The ookinete production in Anopheles stephensi mosquitoes was reduced by 30-40% as compared to wild type parasites. Ookinetes had a reduced capacity to develop into (mature) oocysts.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-204
Ookinetes had a reduced capacity to develop into (mature) oocysts. At two days after feeding of mosquitoes the number of young oocysts (stained with anti-P28 antibodies) was reduced by 70-90% when compared to wild type. At day 12 after feeding the oocysts numbers were reduced by 65-85%. Day 12 oocysts were significantly smaller than wild type oocysts and no formation of sporozoites was observed.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-204
No formation of sporozoites
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Localization of Plasmodium falciparum c-glutamylcysteine synthetase–green fluorescent protein (PfcGCSGFP) and glutathione synthetase-GFP (PfGS-GFP). P. falciparum GS and gGCS C-terminally tagged with GFP were expressed in P. falciparum D10 erythrocytic stages. Fluorescence was analyzed using an Axioskop-2 mot plus microscope (Zeiss) equipped with a Hamamatsu C4742-95 CCD camera and was shown to be present throughout the cytoplasm of both transfected parasite lines, suggesting that GSH biosynthesis is cytoplasmic. No fluorescence is seen in the digestive vacuoles (DV) of the parasites or the erythrocyte host cell (E). E, erythrocyte; P, parasite; DV, digestive vacuole with hemozoin crystals.Patzewitz EM, Salcedo-Sora JE, Wong EH, Sethia S, Stocks PA, Maughan SC, Murray JA, Krishna S, Bray PG, Ward SA, Müller S. Glutathione Transport: A New Role for PfCRT in Chloroquine Resistance. Antioxid Redox Signal. 2012 19(7):683-95 PMID:

See original on MMP

More information

PlasmoDB PKNH_0716900
GeneDB PKNH_0716900
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK9_1750w, PKH_071610
Orthologs PBANKA_0819800 , PCHAS_0820100 , PF3D7_0918900 , PVP01_0717300 , PVX_099360 , PY17X_0823100
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