Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-960 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
Difference from wild-type
RMgm-960
The course of infections was similar to wild type parasites. Slightly reduced schizont maturation in in vitro cultures.
Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-960
Normal production of mature male and female gametocytes. Strongly reduced (85-90%) formation of male gametes (exflagellation)
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-960
Normal production of mature male and female gametocytes. Strongly reduced (85-90%) formation of male gametes (exflagellation). Frmale fertility strongly reduced. Ookinete formation strongly reduced (~99%)
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-960
Ookinete and oocyst formation strongly reduced (~99%)
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-960
Ookinete,oocyst and sporozoite formation strongly reduced (95-99%)
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

(B) Localization of native PfCuP-ATPase in trophozoite-infected erythrocytes using immuno-fluorescence microscopy: panels 1 and 3, DAPI (4,6-diamidino-2-phenylindole)-fluorescent image; panel 2, pre-immune serum (Texas-Red-labelled cells); panel 4, (Texas-Red-labelled cells) affinity-purified polyclonal antibody. The brightest signals were observed in late trophozoite and schizont stages. Signal was competed with MBP–MBD (fusion protein MBP–MBD containing the metal-binding motif Met-Thr-Cys-Asp-Asn-Cys of PfCuP-ATPase) and not MBP alone. (C) Trophozoite/schizont-infected erythrocytes were stained with antibody against PfCuP-ATPase using a gold pre-embedding immunoelectron microscopic method. The arrows indicate signals both on the parasite as well as on the erythrocyte membrane. The scale bar is 1 μm.Reproduced with permission, from Rasoloson D, Shi L, Chong CR, Kafsack BF, Sullivan DJ. Copper pathways in Plasmodium falciparum infected erythrocytes indicate an efflux role for the copper P-ATPase. Biochem J. 2004 381:803-11. © the Biochemical Society.

See original on MMP

More information

PlasmoDB PKNH_0702600
GeneDB PKNH_0702600
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK9_0210c, PKH_070210
Orthologs PBANKA_0416500 , PCHAS_0417400 , PF3D7_0904900
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