Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-120 Imported from RMgmDB
P. falciparum 3D7
Possible
17950739 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-120 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-120 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-120 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-120
Normal numbers of oocysts are produced. Sporozoite formation within the oocysts is blocked. The diameters of oocysts were significantly larger than that of wild type on days 14 and 21 of infection. Light microscopy revealed the presence of two distinct populations of oocysts: those that displayed a phenotype reminiscent of immature wild type oocysts (i.e., non-sporulated), and those that appeared vacuolated/degenerate compared to wild type.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-120
No midgut sporozoites were observed on day 10/11 p.i. By day 18 p.i., reduced numbers (typically 0%12%) of sporozoites were observed in dissected midguts. The number of sporozoites in salivary gland preparations infections was consistently reduced to <1% of wild type. The expression and targeting of the circumsporozoite protein in midgut sporozoites was indistinguishable from that in wild type.To test if the observed sporozoites were infectious to mice, infected mosquitoes were allowed to feed on mice on days 21 and 28 p.i. Blood stage parasites were observed in all mice bitten by wild type-infected mosquitoes when screened on day 4/5 post-bite. In contrast, mice bitten by mutant-infected mosquitoes remained uninfected.
Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-120 Imported from RMgmDB

More information

PlasmoDB PKNH_0701500
GeneDB PKNH_0701500
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK9_0100w, PKH_070100
Orthologs PBANKA_0417600 , PCHAS_0418500 , PF3D7_0903800 , PVP01_0702200 , PVX_098635 , PY17X_0420400
Google Scholar Search for all mentions of this gene