Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Immunofluorescence assays for localization of PfRRF2.A. Live cell imaging of the PfRRF2leader–GFP transfectant with MitoTracker indicates predominantly mitochondrial targeting of PfRRF2. C. Immunofluorescence localization using anti-GFP Ab and MitoTracker shows colocalization of the PfRRF2leader–GFP signal with mitochondrion.D. Absence of overlap between the apicoplast marker ACP and PfRRF2leader–GFP indicating that the PfRRF2 leader does not target the protein to the apicoplast. E. Immunofluorescence localization of PfRRF2 in P. falciparum 3D7-infected erythrocytes using rabbit anti-RRF2 antibody as probe. Cellswere stained with MitoTracker prior to fixation. F. P. falciparum D10 ACPleader–GFP infected erythrocytes were probed with anti-GFP and anti-RRF2 Abs. PfRRF2 signal did not overlap with the apicoplast.Gupta A, Mir SS, Jackson KE, Lim EE, Shah P, Sinha A, Siddiqi MI, Ralph SA,Habib S. Recycling factors for ribosome disassembly in the apicoplast andmitochondrion of Plasmodium falciparum. Mol Microbiol. 2013 88(5):891-905 .

See original on MMP

More information

PlasmoDB PKNH_0513200
GeneDB PKNH_0513200
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK4_1650w, PKH_052470
Orthologs PBANKA_0521800 , PCHAS_0522000 , PF3D7_0420400 , PVP01_0529900 , PVX_090115 , PY17X_0523200
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