Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Possible
RMgm-617 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-516 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-515 Imported from RMgmDB
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
20197405
\"Targeted disruption of the PfPuf2 gene did not affect asexual growth of the parasite, but promoted the formation of gametocytes and differentiation of male gametocytes. Complementation studies were performed to confirm that the resultant phenotypic changes were due to disruption of the PfPuf2 gene. Episomal expression of PfPuf2 under its cognate promoter almost restored the gametocytogenesis rate in a PfPuf2 disruptant to the level of the wild-type parasite. It also partially restored the effect of PfPuf2 disruption on male-female sex ratio. In addition, episomal overexpression of PfPuf2 under its cognate promoter but with a higher concentration of the selection drug or under the constitutive hsp86 promoter in both the PfPuf2-disruptant and wild-type 3D7 lines, further dramatically reduced gametocytogenesis rates and sex ratios. These findings suggest that in this early branch of eukaryotes the function of PfPuf2 is consistent with the ancestral function of suppressing differentiation proposed for Puf-family proteins.\"
Theo Sanderson, Francis Crick Institute
P. yoelii yoelii 17X
Possible
RMgm-842 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-617 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-516 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-515 Imported from RMgmDB
P. falciparum 3D7 Asexual
No difference
20197405
\"Targeted disruption of the PfPuf2 gene did not affect asexual growth of the parasite, but promoted the formation of gametocytes and differentiation of male gametocytes. Complementation studies were performed to confirm that the resultant phenotypic changes were due to disruption of the PfPuf2 gene. Episomal expression of PfPuf2 under its cognate promoter almost restored the gametocytogenesis rate in a PfPuf2 disruptant to the level of the wild-type parasite. It also partially restored the effect of PfPuf2 disruption on male-female sex ratio. In addition, episomal overexpression of PfPuf2 under its cognate promoter but with a higher concentration of the selection drug or under the constitutive hsp86 promoter in both the PfPuf2-disruptant and wild-type 3D7 lines, further dramatically reduced gametocytogenesis rates and sex ratios. These findings suggest that in this early branch of eukaryotes the function of PfPuf2 is consistent with the ancestral function of suppressing differentiation proposed for Puf-family proteins.\"
Theo Sanderson, Francis Crick Institute
P. yoelii yoelii 17X Asexual
No difference
RMgm-842 Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-617
Increased numbers of (male) gametocytes
Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-516 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-515 Imported from RMgmDB
P. falciparum 3D7 Gametocyte
Difference from wild-type
20197405
\"Targeted disruption of the PfPuf2 gene did not affect asexual growth of the parasite, but promoted the formation of gametocytes and differentiation of male gametocytes. Complementation studies were performed to confirm that the resultant phenotypic changes were due to disruption of the PfPuf2 gene. Episomal expression of PfPuf2 under its cognate promoter almost restored the gametocytogenesis rate in a PfPuf2 disruptant to the level of the wild-type parasite. It also partially restored the effect of PfPuf2 disruption on male-female sex ratio. In addition, episomal overexpression of PfPuf2 under its cognate promoter but with a higher concentration of the selection drug or under the constitutive hsp86 promoter in both the PfPuf2-disruptant and wild-type 3D7 lines, further dramatically reduced gametocytogenesis rates and sex ratios. These findings suggest that in this early branch of eukaryotes the function of PfPuf2 is consistent with the ancestral function of suppressing differentiation proposed for Puf-family proteins.\"
Theo Sanderson, Francis Crick Institute
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-842 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-617 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-516 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-515 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-842 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-617 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-516 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-515 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-842 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-617
Normal numbers of oocysts and salivary gland sporozoites are formed. Sporozoites show a strongly reduced infectivity to mice both after intravenous inoculation of sporozoites or after infection by mosquito bite. Sporozoites showed normal invasion of HepG2 cells in vitro. However, both in vivo and in vitro liver stage development was strongly affected.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-516
Normal numbers of oocysts and salivary gland sporozoites are formed. Sporozoites are defective in gliding motility and hepatocyte cell traversal in vitro (see also 'Additional remarks phenotype' for further description of the sporozoites). Sporozoites are less infective to hepatocytes in vitro and to mice after intravenous inoculation of sporozoites. Sporozoites could not be transmitted to naive mice by mosquito bite.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-515
Normal numbers of oocysts and salivary gland sporozoites are formed. Sporozoites are defective in gliding motility and hepatocyte cell traversal in vitro (see also 'Additional remarks phenotype' for further description of the sporozoites). Sporozoites are less infective to hepatocytes in vitro and to mice after intravenous inoculation of sporozoites. Sporozoites could not be transmitted to naive mice by mosquito bite.
Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
Difference from wild-type
RMgm-842
Normal numbers of salivary gland sporozoites.Sporozoites progressively lost infectivity after arrival in the salivary glands, becoming non-infectious within the following 8 days. A significant and progressively increasing fraction of sporozoites prematurely transformed inside the salivary glands into forms that resemble young liver stages
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-617
Normal numbers of oocysts and salivary gland sporozoites are formed. Sporozoites show a strongly reduced infectivity to mice both after intravenous inoculation of sporozoites or after infection by mosquito bite. Sporozoites showed normal invasion of HepG2 cells in vitro. However, both in vivo and in vitro liver stage development was strongly affected.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-516
Normal numbers of oocysts and salivary gland sporozoites are formed. Sporozoites are defective in gliding motility and hepatocyte cell traversal in vitro. Sporozoites are less infective to hepatocytes in vitro and to mice after intravenous inoculation of sporozoites. Sporozoites could not be transmitted to naive mice by mosquito bite.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-515
Normal numbers of oocysts and salivary gland sporozoites are formed. Sporozoites are defective in gliding motility and hepatocyte cell traversal in vitro. Sporozoites are less infective to hepatocytes in vitro and to mice after intravenous inoculation of sporozoites. Sporozoites could not be transmitted to naive mice by mosquito bite.
Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-842
Normal numbers of salivary gland sporozoites.Sporozoites progressively lost infectivity after arrival in the salivary glands, becoming non-infectious to mice within the following 8 days.
Imported from RMgmDB

More information

PlasmoDB PKNH_0509500
GeneDB PKNH_0509500
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) PK4_1290c, PKH_052120
Orthologs PBANKA_0719200 , PCHAS_0728200 , PF3D7_0417100 , PVP01_0526500 , PVX_089945 , PY17X_0719200
Google Scholar Search for all mentions of this gene