Last updated 1 year ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
18452584 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
RMgm-308 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-4512 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Gametocyte
Attenuated
18452584 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
No difference
RMgm-308 Imported from RMgmDB
P. yoelii yoelii 17X Asexual
No difference
RMgm-4512 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-308 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-4512 Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-308
Gametocyte production and fertilisation were not different from wild type. During the first 12h of in vitro culture, mutant zygotes differentiated into morphologically advanced ookinetes (stage IV-VI) in similar numbers as wild type. In cultures of wild ookinetes, mature ookinete forms continued to accumulate in in vitro culture, reaching 60% of all macrogamete-derived parasites by 24h, the remainder presumably being unfertilised macrogametes. In contrast, all the morphologically mature ookinetes in the early cultures (12h) of the mutant were replaced successively by stumpy and round forms and at 24h, hardly any typically shaped ookinetes were present. Motility of ookinetes was affected (see 'Additional information'). The mutant ookinetes had a greatly reduced capacity to develop into oocysts; oocyst numbers on the mosquito midgut epithelium were reduced by >94%.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-308
The mutant ookinetes had a greatly reduced capacity to develop into oocysts; oocyst numbers on the mosquito midgut epithelium were reduced by >94%.
Imported from RMgmDB

More information