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Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
36400305
\"\"We show using C-terminal truncations and gene knockouts of each of Pfczif1 and Pfczif2 that neither are essential for blood stage growth. However, they could not both be knocked out simultaneously, suggesting that at least one is needed for parasite growth in vitro. Immunofluorescence localisation of PfCZIF1 and PfCZIF2 indicated that both proteins occur in discrete foci on the periphery of the parasite\\\'s cytosol and biochemical assays suggest they are peripherally associated to a membrane.\"
Theo Sanderson, Francis Crick Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Possible
36400305
\"\"We show using C-terminal truncations and gene knockouts of each of Pfczif1 and Pfczif2 that neither are essential for blood stage growth. However, they could not both be knocked out simultaneously, suggesting that at least one is needed for parasite growth in vitro. Immunofluorescence localisation of PfCZIF1 and PfCZIF2 indicated that both proteins occur in discrete foci on the periphery of the parasite\\\'s cytosol and biochemical assays suggest they are peripherally associated to a membrane.\"
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

Immunofluorescence analysis of schizonts, segmenter, and released merozoites of P. falciparum using anti-D13 MAb DD1.1 and confocal microscopy. In column I, staining with MAb DD1.1 is shown, while in column II the phase contrast of the corresponding parasites is presented. Specific staining of schizonts (first row), segmenters (second row) and released merozoites (third row) are presented. Interestingly, in the merozoite stage, D13 protein seemed to be concentrated in a distinct area of the cell, while during schizont stages the protein was more evenly distributed in the parasite.Daubenberger CA, Diaz D, Curcic M, Mueller MS, Spielmann T, Certa U, Lipp J, Pluschke G. Identification and characterization of a conserved, stage-specific gene product of Plasmodium falciparum recognized by parasite growth inhibitory antibodies. Infect Immun. 2003 71(4):2173-81.

See original on MMP

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