|P. falciparum 3D7||Asexual||
| 29582546 (Knock down)
glmS knockdown gave 90% reduction of parasitaemia after two cycles. No merozoites formed in 50% of schizonts, despite normal nuclear division. Parasites could not recover if knockdown was removed after the onset of schizogony. Trafficking was severely disrupted (see details in paper).
|Theo Sanderson, Wellcome Trust Sanger Institute|
The putative protein sorting receptor PfSortilin localizes to the P. falciparum Golgi apparatus. Co-localization of PfSortilin-HA and ERD2 in aldehyde-fixed clone C9 parasites. T, trophozoite; Sz, schizont. Scale bar, 2 mm. In trophozoite- and schizont-stage parasites, PfSortilin-HA localized to puncta that were also recognized by antibodies to the Golgi marker ERD2 (Fig. 4C). In ring-stage parasites, PfSortilin-HA could not be detected above non-specific background, even though the Golgi apparatus was present as determined by ERD2 immunolocalization.Krai P, Dalal S, Klemba M. Evidence for a Golgi-to-Endosome Protein Sorting Pathway in Plasmodium falciparum. PLoS One. 2014 9(2):e89771. PMID:See original on MMP