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Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
18437218 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Refractory
RMgm-807 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
36238365 (Knock down)
\" Based on these associations, we studied the role of PfHDP in Hb uptake using the PfHDP‐HA‐GlmS transgenic parasites line. PfHDP knockdown significantly reduced the Hb uptake in these transgenic parasites in comparison to the wild‐type parasites. Morphological analysis of PfHDP‐HA‐GlmS transgenic parasites in the presence of GlcN showed food vacuole abnormalities and parasite stress, thereby causing a growth defect in the development of these parasites. Transient knockdown of a member of translocon complex, PfHSP101 in HSP101‐DDDHA parasites also showed a decreased uptake of Hb inside the parasite. Together, these results advocate an interaction between PfHDP and the translocon complex at the parasitophorus vacuole membrane and also suggest a role for PfHDP in the uptake of Hb and parasite development.\"
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

Circuitous transport and delivery of HDP to the FV. A, HDP is secreted into the cytosol of infected erythrocytes (arrowhead) in early ring stages before any Hz could be detected inside the parasite. B, After secreting it into the host cell cytosol, parasite intakes HDP through the cytostome. C, HDP could be found in transport vesicles destined to the FV. D, Transport vesicles deliver HDP to the FV. E, HDP (arrows) was found in close proximity of Hz crystals. F, HDP (18 nm particles) is transported to FV along with hemoglobin (12 nm particles). Inset G, HDP is being internalized along with hemoglobin. Inset H, Transport vesicle ready to deliver both, HDP and hemoglobin, to the FV. cyt, cytostome; fv, food vacuole; fvm, fv membrane; hz, hemozoin; hdp, heme detoxification protein; hb, hemoglobin; nu, nucleus; par, parasite; ppm, parasite plasma membrane; pvm, parasitophorous vacuole membrane; irbc, infected red blood cell; rbcm, RBC membrane; tv, transport vesicle. Bar, 0.5 mm.Jani D, Nagarkatti R, Beatty W, Angel R, Slebodnick C, Andersen J, Kumar S, Rathore D. HDP-a novel heme detoxification protein from the malaria parasite. PLoS Pathog. 2008 4(4):e1000053.

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Left column: Immuno-fluorescence staining using anti-HDP antibodies showed that transfected parasites continually expressed HDP (green). Arrows on the DIC image indicate hemozoin. Parasite nucleus (blue) is stained with DAPI. Right column: Non-specific recombination of HDP-YFP chimera. Immuno-fluorescence staining of with anti HDP antibodies also indicated the expression of the native HDP. Arrows on the DIC image indicate hemozoin. Parasite nucleus (blue) is stained with DAPI. Jani D, Nagarkatti R, Beatty W, Angel R, Slebodnick C, Andersen J, Kumar S, Rathore D. HDP-a novel heme detoxification protein from the malaria parasite. PLoS Pathog. 2008 4(4):e1000053.

See original on MMP

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