Localization of PfApyrase-GFP via live cell imaging. a The parasites werestained with HOECHST 33342 and ER-Tracker™Red BODIPYTR to visualize the nucleus and the ER, respectively. Different developmental stages of intraerythrocytic P. falciparum are shown to demonstrate the different localizations. The white arrows indicate the localization of the digestive vacuole within P. falciparum. As can be seen, the P. falciparum apyrase isexpressed throughout the asexual cycle. However, its localization changes as the parasite develops. In the ring and trophozoite stages, the P. falciparum apyrase co-localizes with the endoplasmic reticulum, also surrounding the parasite nucleus. As the parasite grows and forms several merozoites, the enzyme changes itslocalization being translocated to the digestive vacuole.Borges-Pereira L, Meissner KA, Wrenger C, Garcia CR. Plasmodium falciparum GFP-E-NTPDase expression at the intraerythrocytic stages and its inhibition blocks the development of the human malaria parasite. Purinergic Signal. 2017 Mar 11. PMID: 28285440