Last updated 2 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
33537025
\"The knockout of PfAP2-G2 led to a ~95% decrease in the number of mature gametocytes with a more substantial influence on the production and maturation of the male gametocytes, resulting in a higher female/male gametocyte ratio. \"
Theo Sanderson, Francis Crick Institute
P. berghei ANKA
Possible
RMgm-4102 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1341 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1340 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1022 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM
P. yoelii yoelii 17X
Possible
RMgm-4384 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Possible
33368818 (Conditional)
\" Disruption of pfap2-g2 does not impact asexual development, but the majority of sexual parasites are unable to mature beyond stage III gametocytes. The absence of pfap2-g2 leads to overexpression of 28% of the genes bound by PfAP2-G2 and none of the PfAP2-G2 bound genes are downregulated, suggesting that it is a repressor. We also find that PfAP2-G2 interacts with chromatin remodeling proteins, a microrchidia (MORC) protein, and another ApiAP2 protein (PF3D7_1139300). Overall our data demonstrate that PfAP2-G2 establishes an essential gametocyte maturation program in association with other chromatin-related proteins.\"
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Gametocyte
Difference from wild-type
33537025
\"The knockout of PfAP2-G2 led to a ~95% decrease in the number of mature gametocytes with a more substantial influence on the production and maturation of the male gametocytes, resulting in a higher female/male gametocyte ratio. \"
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
No difference
RMgm-4102 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1341 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1340 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1022 Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. yoelii yoelii 17X Asexual
No difference
RMgm-4384 Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-4102
Strongly reduced gametocyte production; absence of (male) gametocyte production (exflagellation).
Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-1341
No mature male or female gametocytes are formed. No exflagellation. Evidence is presented that sexually committed parasites (trophozoites) are formed within red blood cells but that these parasites abort development before morphological features of sexual differentiation are visible at the light-microscopic level.
Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-1340
No mature male or female gametocytes are formed. No exflagellation. Evidence is presented that sexually committed parasites (trophozoites) are formed within red blood cells but that these parasites abort development before morphological features of sexual differentiation are visible at the light-microscopic level.
Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-1022
Reduced gametocyte production (95% reduction).
Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
Difference from wild-type
RMgm-4384
few gametocytes formed
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-4102
Strongly reduced gametocyte production; absence of (male) gametocyte production (exflagellation). No ookinete production
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-1341
No mature male or female gametocytes are formed. No exflagellation. No oocysts are formed.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-1340
No mature male or female gametocytes are formed. No exflagellation. No oocysts are formed.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-1022
Reduced gametocyte production (95% reduction). No mosquito transmission was observed
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4102
Strongly reduced gametocyte production; absence of (male) gametocyte production (exflagellation). No ookinete production. No oocyst production.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1341
No mature male or female gametocytes are formed. No exflagellation. No oocysts are formed.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1340
No mature male or female gametocytes are formed. No exflagellation. No oocysts are formed.
Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
Difference from wild-type
RMgm-4384
few gametocytes formed; few oocyst produced
Imported from RMgmDB

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