Last updated 7 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
15491999 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
RMgm-808 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-314 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-315 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-316 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
15491999 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
Difference from wild-type
RMgm-808
Asexual blood stages show a (slightly) reduced growth rate and reduced hemozoin formation.
Imported from RMgmDB
P. berghei ANKA Asexual
Difference from wild-type
RMgm-316
Asexual blood stages show a slightly reduced growth rate and show a virulence-attenuated phenotype in mice (see 'Additional remarks phenotype').
Imported from RMgmDB
P. berghei ANKA Asexual
Difference from wild-type
RMgm-314
Asexual blood stages show a slightly reduced growth rate and show a virulence-attenuated phenotype in mice (see 'Additional remarks phenotype').
Imported from RMgmDB
P. berghei ANKA Asexual
Difference from wild-type
RMgm-315
Asexual blood stages show a slightly reduced growth rate and show a virulence-attenuated phenotype in mice (see 'Additional remarks phenotype').
Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
No difference
RMgm-316 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-316 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-316 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-316 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-316 Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Immunolocalization of FP-2 by confocal microscopy. Erythrocytes infected with P. falciparum trophozoites were smeared on a glass slide, fixed with ice-cold methanol, and incubated with polyclonal FP-2 (A) or SERP (B) antibodies followed by Alexa 488 (green) or Alexa 594 (red) conjugated goat anti-rabbit IgG. Merged images of differential interference contrast (DIC) and fluorescence microscopy are shown on the right. C and D, slides were incubated with polyclonal FP-2 together with either plasmepsin IV (C) antibodies followed by Alexa-488-conjugated (green) goat anti-rabbit IgG and Alexa 594-conjugated (red) goat anti-mouse IgG. Merged images of green and red micrographs are shown on the right. The FP-2 antibody stained the entire trophozoite and vesicles or vesicle-like structures of unknown identity were seen extending into the erythrocyte cytoplasm. Co-staining of infected erythrocytes with antibodies to plasmepsin IV, which is present exclusively in the food, confirmed that FP-2 is present in the food vacuole as well as in the area that lies outside of the food vacuole (C). A comparison of FP-2 and SERP staining shows that the FP-2 is localized in the PVM as well as in a compartment that is distinct from the PVM.Dhawan S, Dua M, Chishti AH, Hanspal M. Ankyrin peptide blocks falcipain-2-mediated malaria parasite release from red blood cells. J Biol Chem. 2003 278:30180-6

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Plm-IV localization assay. (a) Confocal microscopy images show that Plm-IV co-localizes with the dark hemozoin-containing DV in healthy 3D7 P. falciparum parasites but not in (b) parasites treated with 3 mM of CQ for 4 h, where Plm-IV staining is less intense and is found throughout the parasite cytoplasm. (Scale bar set at 5 mm).Ch'ng JH, Lee YQ, Gun SY, Chia WN, Chang ZW, Wong LK, Batty KT, Russell B, Nosten F, Renia L, Tan KS. Validation of a chloroquine-induced cell death mechanism for clinical use against malaria. Cell Death Dis. 2014 Jun 26;5:e1305.

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