Last updated 3 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
30235972 Theo Sanderson, Google AI
P. falciparum 3D7
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Transcriptional effect
30235972 (Conditional)
Di-cre approach with loxP-intron. Incomplete deletion, an increase in especially antisense transcripts. Likely a substantial growth reduction given failure to obtain clones by limiting dilution.
Theo Sanderson, Google AI
P. falciparum 3D7 Asexual
Difference from wild-type
32079470 (Knock down)
'by gene knock-down strategy, we showed that the cooperation of RNA exosome catalytic subunit PfDis3 and PfMtr4 cofactor played a major role in ns-ncRNAs degradation'
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

Coimmunofluorescence assay of three plasmodial exoribonucleases and a core exosome member, PfRrp45, in ring-stage wild-type 3D7 parasites. The rabbit anti-PfRrp45, anti-PfRNase II, anti-PfRrp6 and mouse anti-PfDis3 antibodies were used in these assays. DAPI, 49,6-diamidino-2-phenylindole. Scale bar, 1 mm. Immunofluorescence assay (IFA) analysis revealed that PfRrp6 and PfRNase II localize as distinct foci at the nuclear periphery and that PfDis3 is localized in the cytoplasm adjacent to the nucleus at the ring stage .Zhang Q, Siegel TN, Martins RM, Wang F, Cao J, Gao Q, Cheng X, Jiang L, Hon CC, Scheidig-Benatar C, Sakamoto H, Turner L, Jensen AT, Claes A, Guizetti J, Malmquist NA, Scherf A. Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria. Nature. 2014 Jun 29. PMID:

See original on MMP

More information

PlasmoDB PF3D7_1359300
GeneDB PF3D7_1359300
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) MAL13P1.289
Orthologs PBANKA_1135600 , PCHAS_1135100 , PKNH_1112000 , PVP01_1112600 , PVX_114935 , PY17X_1137100
Google Scholar Search for all mentions of this gene