SET-domain proteins PF08_0012 and Histone-lysine N-methyltransferase PF13_0293 localize to the nuclear periphery marked by CenPA. (A) Localization of PF08_0012 reveals a clustered localization pattern present throughout the nuclear periphery of the cell. PF08_0012 is HA-tagged at the C-terminus and detected with anti-HA antibodies. (B) Live imaging of PF13_0293 shows localization to a distinct area in the nuclear periphery. Co-localization studies by immunofluorescence are shown. Nup100 (PFI0250c) and CenPA (histone H3 PF13_0185) are used as markers of the nuclear periphery.Volz J, Carvalho TG, Ralph SA, Gilson P, Thompson J, Tonkin CJ, Langer C, Crabb BS, Cowman AF. Potential epigenetic regulatory proteins localise to distinct nuclear sub-compartments in Plasmodium falciparum. Int J Parasitol. 2010 40:109-21. Copyright Elsevier 2010.

Schematic representation of the Plasmodium falciparum nuclear architecture. A schematic representation of an infected red blood cell (RBC) and an enlargement of the P. falciparum nucleus are shown. From the centre towards the periphery we have defined three distinct areas (1–3) in which nuclear proteins specifically distribute. A summary is shown in which trophozoite stages have been analyzed. Proteins, previously predicted to functionally interact, are shown in red. Proteins previously predicted for their histone target sites are indicated by grey boxes. A, apicoplast. M, mitochondria, No, nucleolus.Volz J, Carvalho TG, Ralph SA, Gilson P, Thompson J, Tonkin CJ, Langer C, Crabb BS, Cowman AF. Potential epigenetic regulatory proteins localize to distinct nuclear sub-compartments in Plasmodium falciparum. Int J Parasitol. 2010 40(1):109-21. Copyright Elsevier.