Localization of PfCK2 subunits over the life cycle of the bloodstage parasite (A-C) Hemagglutinin (HA)-tagged PfCK2 subunits (green) localized to both nuclear and cytoplasmic compartments in rings, trophozoites and schizonts. The limits of the parasitophorous vacuole membrane (PVM) are marked by Exp-2 (red). (D) HA-tagged PfCK2 subunits were detected in both the cytoplasmic and nuclear fraction. A signal for all three subunits was detectable in both the cytoplasmic and nuclear compartments of the parasite.Dastidar EG, Dayer G, Holland ZM, Dorin-Semblat D, Claes A, Chene A, Sharma A, Hamelin R, Moniatte M, Lopez-Rubio JJ, Scherf A, Doerig C. Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway. BMC Biol. 2012 Jan 31;10(1):5.

Immuno-electron microscopy of the hemagglutinin (HA)-tagged PfCK2α and PfCK2β2 subunits. Immuno-electron microscopy was performed with an anti-HA antibody on ring-, trophozoite- and schizont-stage parasites from the HA-tagged lines. HAPfCK2α and HA-PfCK2β2 localized to both cytoplasmic and nuclear compartments in accordance with our IFA and Western-blot data.Dastidar EG, Dayer G, Holland ZM, Dorin-Semblat D, Claes A, Chene A, Sharma A, Hamelin R, Moniatte M, Lopez-Rubio JJ, Scherf A, Doerig C. Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway. BMC Biol. 2012 Jan 31;10(1):5.