PhenoPlasm

Species	Disruptability	Reference	Submitter
P. falciparum 3D7	Possible	20472690	Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7	Possible	USF piggyBac screen (Insert. mut.)	USF PiggyBac Screen

P. berghei ANKA	Possible	RMgm-673	Imported from RMgmDB
P. berghei ANKA	Possible	RMgm-671	Imported from RMgmDB
P. berghei ANKA	Possible	RMgm-224	Imported from RMgmDB

Species	Stage	Phenotype	Reference	Submitter
P. falciparum 3D7	Asexual	No difference	20472690	Theo Sanderson, Wellcome Trust Sanger Institute

P. berghei ANKA	Asexual	Difference from wild-type	RMgm-673 msp7 infected BALB/c mice showed only a mild delay in the parasite growth rate during the initial phase of infection. The mice progressively developed a high parasitemia and died during the third week post infection similarly to wild type-infected mice.In C57BL/6 mice, msp7 parasites showed a significant reduction of the growth rate up to day 12 post infection and failed to induce signs and symptoms of ECM. These mice showed a prolonged course of infection with increasing parasitemia and died during the fourth week post infection with a peak parasitemia reaching 70%. All wild type-infected mice died at around day 8 post-infection when the parasitemia was still low (around 20%) developing a typical ECM pathology. The virulence attenuated phenotype of msp7 parasites varied with the mouse strain used. A substantial fraction of infected msp7 CD1 mice (about 60%) died within 10 days post-infection with signs and symptoms of ECM and with a parasitemia below 15% while the others mice died later of severe anemia.	Imported from RMgmDB
P. berghei ANKA	Asexual	Difference from wild-type	RMgm-671 Mutant blood stages show a minor growth defect resulting in a lower growth rate in BALB/c mice during the initial phase of infection (day 8/10). At later stages of infection (when reticulocytes are prevalent), the growth rate was similar to that of wild type parasites. In aged WISTAR rats (15 weeks) the mutant blood stages showed a reduced growth and virulence phenotype.	Imported from RMgmDB
P. berghei ANKA	Asexual	Difference from wild-type	RMgm-224 Mutants show a minor growth defect resulting in a lower growth rate in Balb/c mice during the initial phase of infection. When reticulocytes were prevalent (after day 9 after infection), the growth rate was similar to that of wild type parasites	Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Colocalization of PfMSRP-1 and PfMSRP-2 with P. falciparum MSP-1 as demonstrated by immunofluorescence of thin blood smears of asynchronous cultures. (a) Slides were incubated with a mouse anti-PfMSRP-1 serum and a fluorescein isothiocyanate-labeled rabbit anti-mouse IgG secondary antibody. (b and f) Slides were incubated with a guinea pig anti-Pf83a serum and a rhodamine-labeled donkey anti-guinea pig IgG secondary antibody. (c) Overlay of panels a and b. (d) Bright-field image of panels a, b, and c. (e) Slides were incubated with a mouse anti-PfMSRP-2 serum and a fluorescein isothiocyanate-labeled rabbit anti-mouse IgG secondary antibody. (g) Overlay of panels e and f. (h) Bright-field image of panels e, f, and g. PfMSRP-1 and PfMSRP-2 were localized to the membranes of trophozoites (a and e). This is similar to the pattern observed with the amino-terminal portion of PfMSP-1 (b and f).Mello K, Daly TM, Morrisey J, Vaidya AB, Long CA, Bergman LW. A multigene family that interacts with the amino terminus of plasmodium MSP-1 identified using the yeast two-hybrid system. Eukaryot Cell. 2002 1(6):915-25.

See original on MMP

Co-localization of MSP7 and MSRP2. Late-stage parasites were formaldehyde-fixed, acetone-permeabilised and reacted with mAb 2G10, (mouse monoclonal anti-MSP7 antibody) and rabbit polyclonal anti-MSRP2 antibodies in an immunofluorescence assay.Kadekoppala M, Ogun SA, Howell S, Gunaratne RS, Holder AA. A systematic genetic analysis of the Plasmodium falciparum MSP7-like family reveals differences in protein expression, location and importance in asexual growth of the blood stage parasite. Eukaryot Cell. 2010 9:1064-74.

See original on MMP

PlasmoDB	PF3D7_1334800
GeneDB	PF3D7_1334800
Malaria Metabolic Pathways	Localisation images Pathways mapped to
Previous ID(s)	MAL13P1.174
Orthologs	PBANKA_1349100 , PCHAS_1353700 , PKNH_1266000 , PVP01_1219700 , PVP01_1220000 , PVP01_1220200 , PVX_082655 , PVX_082665 , PVX_082680 , PY17X_1354100
Google Scholar	Search for all mentions of this gene

PF3D7_1334800 MSP7-like protein (MSRP2)

Disruptability [+]

Mutant phenotypes [+]

Imaging data (from Malaria Metabolic Pathways)

More information