PF3D7_1250100 osmiophilic body protein G377 (G377)
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. falciparum 3D7 |
Possible |
18086189 " female gametocytes lacking the full complement of osmiophilic bodies are significantly less efficient both in vitro and in vivo in their emergence from the erythrocytes upon induction of gametogenesis" |
Theo Sanderson, Wellcome Trust Sanger Institute | |
| P. falciparum 3D7 |
Possible |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
| P. berghei ANKA |
Possible |
RMgm-263 | Imported from RMgmDB | |
| P. berghei ANKA |
Possible |
PlasmoGEM (Barseq) | PlasmoGEM | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. falciparum 3D7 | Asexual |
No difference |
18086189 " female gametocytes lacking the full complement of osmiophilic bodies are significantly less efficient both in vitro and in vivo in their emergence from the erythrocytes upon induction of gametogenesis" |
Theo Sanderson, Wellcome Trust Sanger Institute |
| P. falciparum 3D7 | Gametocyte |
Attenuated |
18086189 " female gametocytes lacking the full complement of osmiophilic bodies are significantly less efficient both in vitro and in vivo in their emergence from the erythrocytes upon induction of gametogenesis" |
Theo Sanderson, Wellcome Trust Sanger Institute |
| P. berghei ANKA | Asexual |
No difference |
RMgm-263 | Imported from RMgmDB |
| P. berghei ANKA | Asexual |
No difference |
PlasmoGEM (Barseq) | PlasmoGEM |
| P. berghei ANKA | Gametocyte |
Difference from wild-type |
RMgm-263
Production of gametocytes was slightly reduced in clone Pbg377-b (622cl1) compared to WT, while the other clone (600cl1) examined was similar to WT.In female gametocytes typical osmiophilic bodies (OB) were not detected by Transmission Electron Microscopy (TEM) but only tiny osmiophilic vesicles, which were reduced in size compared to OB of WT females. The altered morphology of OB in mutant parasites was confirmed by determining minimum and maximum size values of more than a hundred OB sections present in TEM micrographs of WT and Pbg377 gametocytes. These observations indicate that Pbg377 may have a structural role in defining the shape of female OB. Absence of this protein does not result in a near complete lack of OB, as described for P. falciparum mutant lacking Pfg377, but instead results in a dramatic change in OB morphology.15 min post activation of gametocytes a reduction of approximately 60% in egress of females from the host erythrocyte was observed in mutant parasites compared to the WT, while males emerged normally, as expected. At 20 min, egress of mutant female gametocytes/gametes was comparable to the WT. These observations indicate that in the absence of normal OB, female gametocytes are able to egress from the host erythrocyte, although less efficiently than the WT. |
Imported from RMgmDB |
| P. berghei ANKA | Ookinete |
No difference |
RMgm-263 | Imported from RMgmDB |
| P. berghei ANKA | Oocyst |
No difference |
RMgm-263 | Imported from RMgmDB |
| P. berghei ANKA | Sporozoite |
No difference |
RMgm-263 | Imported from RMgmDB |
| P. berghei ANKA | Liver |
No difference |
RMgm-263 | Imported from RMgmDB |
Imaging data (from Malaria Metabolic Pathways)
In-depth analysis of protease localization. A) IFAs using polyclonal mouse antisera directed against PfSUB1, PfαβH and PfM16 were used to investigate the granular localization of these proteases in merozoite-containing mature schizonts (in green). The merozoites were visualized via immunolabelling with polyclonal rabbit antisera directed against PfMSP1 (in red). PfM16 localized to granular structures in schizonts, which did not coincide with the merozoites B) Immunolabeling of osmiophilic bodies using polyclonal rabbit antisera directed against Pfg377 (in red) was employed to demonstrate an accumulation of PfPM6, labeled with the respective mouse antisera (in green), in these organelles. Hoechst 33342 staining was used to highlight the parasite nuclei (in blue). Bar, 2 μm. Results are representative of two independent experiments. The localization of PfPM6 in female gametocytes was don by co-localization experiments were performed, using rabbit antibodies directed against the osmiophilic body protein Pfg377. The double-labelling assays demonstrated that PfPM6 is present in these organelles.Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 Mar 25. [Epub ahead of print]
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| PlasmoDB | PF3D7_1250100 |
| GeneDB | PF3D7_1250100 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | 2277.t00481, MAL12P1.479, PFL2405c |
| Orthologs | PBANKA_1463000 , PCHAS_1465300 , PKNH_1470100 , PVP01_1467200 , PVX_101400 , PY17X_1465600 |
| Google Scholar | Search for all mentions of this gene |