PF3D7_1227200 potassium channel (K1)
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. falciparum 3D7 |
Refractory |
18218136 | Theo Sanderson, Wellcome Trust Sanger Institute | |
| P. falciparum 3D7 |
Refractory |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
| P. berghei ANKA |
Possible |
RMgm-230 | Imported from RMgmDB | |
| P. berghei ANKA |
Possible |
PlasmoGEM (Barseq) | PlasmoGEM | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. berghei ANKA | Asexual |
Difference from wild-type |
RMgm-230
Mice infected with mutant parasites rapidly progressed in parasitaemia from day 4. In contrast to wild type-infected mice, however, parasitaemia in mutant-infected mice leveled off at a plateau 9 days after infection, before approaching the parasitaemia of wild type-infected mice at day 14. This delay in parasitaemia was not statistically significant. Mutant-infected mice showed a slightly prolonged survival compared with wild type-infected mice. |
Imported from RMgmDB |
| P. berghei ANKA | Gametocyte |
No difference |
RMgm-230
Normal gametocyte production |
Imported from RMgmDB |
| P. berghei ANKA | Ookinete |
Difference from wild-type |
RMgm-230
Normal ookinete production. |
Imported from RMgmDB |
| P. berghei ANKA | Oocyst |
Difference from wild-type |
RMgm-230
Mosquitoes infected (81 of 89 dissected) with wild type parasites revealed a median oocyst number of 115 per mosquito (25 and 75 percentiles, oocyst numbers 37 and 251). Only two mosquitoes of 112 fed on mutant-infected mice had oocysts in their midguts, thus demonstrating a 98% reduction in the infectivity of mutant parasites during transmission through mosquitoes. |
Imported from RMgmDB |
| P. berghei ANKA | Sporozoite |
No difference |
RMgm-230 | Imported from RMgmDB |
| P. berghei ANKA | Liver |
No difference |
RMgm-230 | Imported from RMgmDB |
Imaging data (from Malaria Metabolic Pathways)
Indirect Immunofluorescence Assay (IFA) was performed using affinity purified anti-PfK1 F4 antibodies. Representative IFAs are shown. Anti-PfK1 IFAs showed no labeling in rings (data not shown) or uninfected RBCs, and increasing RBC membrane labeling in trophozoites and schizonts. The level of labeling increased with parasite maturity and was located at the plasma membrane of the iRBC, in a semi-punctate pattern.Waller KL, McBride SM, Kim K, McDonald TV. Characterization of two putative potassium channels in Plasmodium falciparum. Malar J. 2008 Jan 24;7:19.
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| PlasmoDB | PF3D7_1227200 |
| GeneDB | PF3D7_1227200 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | 2277.t00263, MAL12P1.263, PFL1315w |
| Orthologs | PBANKA_1442000 , PCHAS_1444000 , PKNH_1446600 , PVP01_1445400 , PVX_123990 , PY17X_1444500 |
| Google Scholar | Search for all mentions of this gene |