Last updated 2 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Cell cycle arrest
https://www.biorxiv.org/content/10.1101/755439v1 (Conditional)
'Stalled during schizont development and failed to produce any merozoites so that there was no associated increase in parasitaemia'
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Asexual
Refractory
https://www.biorxiv.org/content/10.1101/755439v1 (Conditional) Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

Localization studies of UBA (E1) and UBC (E2). (A,B) IFA experiments, using different parasite stages, show that PfUBA1 and PfUBC localize mainly to the cytosol. (C) When co-immunostained with plasmepsin V (PMV), a Plasmodium ER membrane protein marker, there was noticeable overlap between the two proteins, suggesting UBA1 recruitment to the ER as well. Both UBC and UBA1 are mainly dispersed throughout the cytoplasm with small aggregations scattered throughout the parasite. UBA1 was co-immuno-stained with PMV, an ER membrane marker, showing noticeable overlap between the two proteins.Chung DW, Ponts N, Prudhomme J, Rodrigues EM, Le Roch KG. Characterization of the Ubiquitylating Components of the Human Malaria Parasite's Protein Degradation Pathway. PLoS One. 2012;7(8):e43477.

See original on MMP

More information

PlasmoDB PF3D7_1225800
GeneDB PF3D7_1225800
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2277.t00249, MAL12P1.249, PFL1245w
Orthologs PBANKA_1440700 , PCHAS_1442700 , PKNH_1445200 , PVP01_1444000 , PVX_123920 , PY17X_1443200
Google Scholar Search for all mentions of this gene