Last updated 2 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Refractory
34152814
(with CRISPR) Sustained growth was only possible in the presence of IPP, indicating essentiality for the apicoplast. \'PfGyrA disruption was accompanied by loss of plastid acyl-carrier protein (ACP) immunofluorescence and the plastid genome. \'
Theo Sanderson, Francis Crick Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Difference from wild-type
34152814
(with CRISPR) Sustained growth was only possible in the presence of IPP, indicating essentiality for the apicoplast. \'PfGyrA disruption was accompanied by loss of plastid acyl-carrier protein (ACP) immunofluorescence and the plastid genome. \'
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

Localization of PfACP in the apicoplast. An immuno-fluorescence assay was performed using anti-PfACP primary antibodies and fluorescein isothiocyanate (FITC)-conjugated secondary antibodies on glass slides containing thin smears of nonsynchronized P. falciparum parasites. E. Panels 1 and 2 show that PfACP (green) is localized to the apicoplast. DAPI was used to stain the nuclei. (F) Apicoplast localization of PfGyrA and PfGyrB. An immunofluorescence assay was performed using the above-described antibodies on glass slides containing thin smears of P. falciparum parasites (mostly late trophozoite stages). In panels 1and 2, PfACP (green) and PfGyrB (red) are colocalized to the apicoplast. In the third and fourth panels, PfGyrA (green) and PfGyrB (red) show colocalization to the apicoplast.Dar MA, Sharma A, Mondal N, Dhar SK. Molecular cloning of apicoplast-targeted Plasmodium falciparum DNA gyrase genes: unique intrinsic ATPase activity and ATP-independent dimerization of PfGyrB subunit. Eukaryot Cell. 2007 6(3):398-412.

See original on MMP

More information

PlasmoDB PF3D7_1223300
GeneDB PF3D7_1223300
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2277.t00224, MAL12P1.224, PFL1120c
Orthologs PBANKA_1438200 , PCHAS_1440200 , PKNH_1442500 , PVP01_1441500 , PVX_123795 , PY17X_1440700
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