Last updated 2 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Cell cycle arrest
32098816 (Conditional)
DiCre approach. "Parasite counts by fluorescence-activated cell sorting (FACS) revealed that induced-KO of pfap2 prevented parasite replication within a single asexual cycle, without appreciable recovery over multiple cycles (Fig. 2D), showing that pfap2 is required for asexual replication in vitro. Importantly, the Cre-mediated endogenous pfap2 KO was fully complemented with an episomally expressed copy of AP-2 -GFP under a constitutive promoter." "When examined by Giemsa staining, parasites lacking pfap2 arrest as malformed schizonts which are still present in the culture at 60 h postinvasion (Fig. 2H). These defective schizonts occupy approximately half of the red cell cytoplasm and contain poorly segmented merozoites compared to wild-type schizonts. Consistent with reduced size, pfap2 KO schizonts carried fewer nuclei (mean standard deviation [KO, 13.0 4.9; wild type, 19.3 4.8; P  0.0001; n  50 KO and 50 wild type]). As determined by FACS, the mean DNA content may be slightly lower in KO schizonts (mean standard deviation [KO, 18,180 8,900 U; wild type, 19,980 10,000 U; P  0.001; n  21,924 events [KO] and 21,552 events [wild type]), but this difference is small and does not explain the more than 45% difference in number of segmented nuclei (Fig. S6). Rarely, rap-treated parasites were observed to undergo egress and invasion, probably due to occasional failure to excise pfap2 ." Widespread defects in merozoite morphology.
Theo Sanderson, Francis Crick Institute

More information

PlasmoDB PF3D7_1218300
GeneDB PF3D7_1218300
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2277.t00177, MAL12P1.177, PFL0885w
Orthologs PBANKA_1433900 , PCHAS_1435900 , PKNH_1437900 , PVP01_1437100 , PVX_123590 , PY17X_1436300
Google Scholar Search for all mentions of this gene