Last updated 8 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
25011111 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
RMgm-1041 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
RMgm-1041 Imported from RMgmDB
P. berghei ANKA Asexual
Attenuated
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-1041
Reduced numbers of macrogametes. The male:female gametocyte ratio is 1:1
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-1041
Ookinete differentiation blocked at stage II.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-1041
None formed
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-1041
None formed
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1041
Not transmitted
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Deconvolution immunofluorescence microscopy of PfNT1 in P. falciparum-infected red blood cells. Parasites were examined by deconvolution microscopy using illumination at 546 nm to visualize PfNT1 (panels A–D), PfHT1 (panel E), and P. falciparum protein phosphatase 2C (panel F) conjugated to the FITC-conjugated anti-rabbit secondary antibody or at 488 nm to visualize Band 3 complexed with the Texas Red-conjugated anti-mouse secondary antibody (all panels). Panel A depicts a ring stage parasite, panel B shows two trophozoites, and schizonts are exhibited in panels C–F. Each panel is accompanied by an inset displaying nuclear staining with Hoechst dye.PfNT1 and PfHT1 are localized to the parasite plasma membrane. Antibodies specific for the cytosolic P. falciparum protein phosphatase 2C enzyme produce a diffuse staining pattern indicative of its cytoplasmic milieu.Rager N, Mamoun CB, Carter NS, Goldberg DE, Ullman B. Localization of the Plasmodium falciparum PfNT1 nucleoside transporter to the parasite plasma membrane. J Biol Chem. 2001 276:41095-9.

See original on MMP

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