Last updated 7 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
27060339 Theo Sanderson, Wellcome Trust Sanger Institute
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
RMgm-892 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
27060339 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Asexual
No difference
RMgm-892 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Ookinete
No difference
RMgm-892 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-892 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-892 Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-892 Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Over-expression and localization of PfDHHC3 and PFDHHC9 (PF11_0167) in late stage parasites. C. Expression of PfDHHC3-mCherry. C. Expression of PfDHHC3-mCherry. C2. Microscopic analysis located this GFP-fusion protein in the periphery of the nascent merozoites. C3. Co-localization with the IMC marker GAPM2 (anti-GAPM2, green) in fixed cells shows differential localization of PfDHHC2-mCherry with the IMC localization in early stages (T1) and colocalization in nascent merozoites (T3) consistent with plasma membrane association. Scale bar, 1 μm. D.Expression of PfDHHC9-mCherry. D2. Microscopy localized this fusion protein mainly in apical structures in the parasite. D3. Co-localization with the IMC marker GAPM2 (anti-GAPM2, green) in fixed cells shows differential localization of PfDHHC9 with the IMC (T3). Scale bar, 1 μm.Wetzel J, Herrmann S, Swapna LS, Prusty D, Peter AT, Kono M, Saini S, Nellimarla S, Wong TW, Wilcke L, Ramsay O, Cabrera A, Biller L, Heincke D, Mossman K, Spielmann T, Ungermann C, Parkinson J, Gilberger TW. The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite. J Biol Chem. 2014 Nov 25.

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Expression and localisation of PfDHHC proteins in P. falciparum schizonts. Triple-HA-tagged PfDHHC proteins were localised by immunofluorescence using antibodies against the 3-HA tag (green). Immunofluorescence staining of each of the tagged PfDHHC proteins was compared against that of the following known localisation markers (red): ERD2 (Golgi marker), BIP (endoplasmic reticulum marker), RAP1 (rhoptry marker), GAP45 (inner membrane complex marker) and MSP1 (plasma membrane marker). Nuclear staining by DAPI is shown in blue. For the staining of PfDHHC9 with GAP45 and MSP1, both a late schizont, as well as an early schizont, is shown in order to differentiate between IMC and plasma membrane localisation. Scale bar: 5 μm.Tay CL, Jones ML, Hodson N, Theron M, Choudhary JS, Rayner JC. Study of Plasmodium falciparum DHHC palmitoyl-transferases identifies a role for PfDHHC9 in gametocytogenesis. Cell Microbiol. 2016 Apr 6.

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Expression and localization of PfDHHC proteins in Plasmodium falciparum schizonts. Triple-HA-tagged PfDHHC proteins were localized by immuno-fluorescence using antibodies against the 3-HA tag (green). Immunofluorescence staining of each of the tagged PfDHHC proteins was compared against that of the following known localization markers (red): ERD2 (Golgi marker), BIP (endoplasmic reticulum marker), RAP1 (rhoptry marker), GAP45 (inner membrane complex marker) and MSP1 (plasma membrane marker). Nuclear staining by DAPI is shown in blue. For the staining of PfDHHC9 with GAP45 and MSP1, a late schizont, as well as an early schizont, is shown in order to differentiate between inner membrane complex and plasma membrane localization. Scale bar: 5 μm.Tay CL, Jones ML, Hodson N, Theron M, Choudhary JS, Rayner JC. Study of Plasmodium falciparum DHHC palmitoyl transferases identifies a role for PfDHHC9 in gametocytogenesis. Cell Microbiol. 2016 18(11):1596-1610.

See original on MMP

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