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Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated
32815516 (Conditional)
Conditional system in which mutant was generated with supplementation so that apicoplast is not required. Gene is required for apicoplast maintenance. Conditional mislocalisation led to decreased transcript levels in the apicoplast.
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

Anti-PfPyKII and anti-P. falciparum acyl carrier protein (ACP) antibodies detected by AlexaFlour goat anti-mouse 594 (red) and goat anti-rabbit 488 (green) secondary antibodies, respectively. Immunofluoresecnce of P. falciparum ACP antibody shows the apicoplast. Merged images indicate co-localization of PfPyKII and P. falciparum ACP. Nucleus stained by DAPI (blue).Maeda T, Saito T, Harb OS, Roos DS, Takeo S, Suzuki H, Tsuboi T, Takeuchi T, Asai T. Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast. This article was published in Parasitol Int. 2009 58:101-5. Copyright Elsevier 2009.

See original on MMP

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