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Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. falciparum 3D7
Possible
36241929
No difference in gam production, exflagellation, but males are infertile due to fertilisation defect
Theo Sanderson, Francis Crick Institute
P. berghei ANKA
Possible
RMgm-156 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-155 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
No difference
36241929
No difference in gam production, exflagellation, but males are infertile due to fertilisation defect
Theo Sanderson, Francis Crick Institute
P. falciparum 3D7 Gametocyte
Difference from wild-type
36241929
No difference in gam production, exflagellation, but males are infertile due to fertilisation defect
Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
No difference
RMgm-156 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-155 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-156
Normal numbers of male and female gametocytes are produced. Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.
Imported from RMgmDB
P. berghei ANKA Gametocyte
Difference from wild-type
RMgm-155
Normal numbers of male and female gametocytes are produced and the sex ratio was comparable to that of wild type gametocytes. Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-156
Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.Mutant males gametes showed flagella motility and adhesion to surrounding erythrocytes, forming exfagellating centers comparable to wild type. However, no successful entering of flagella into female gametes was observed.
Imported from RMgmDB
P. berghei ANKA Ookinete
Difference from wild-type
RMgm-155
Gamete formation was comparable to that of wild type parasites, as determined by light microscopy of exflagellation (male gamete formation) and escape of females from the host erythrocyte.Male gametes are sterile as shown by the lack of fertilisation when the mutant males are crossed with fertile female gametes. Mutant female gametes are fertile as shown by cross-fertilisation with wild type male gametes.Mutant males attach to females but failed to progress from adhesion to membrane fusion and fertilization.No zygotes and ookinetes are formed.
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-156
No ookinete and oocyst formation (see phenotype 'Fertilization and ookinete').
Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-155
No ookinete and oocyst formation (see phenotype 'Fertilization and ookinete').
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-156
No sporozoite formation (see phenotype 'Fertilization and ookinete' and 'Oocyst').
Imported from RMgmDB
P. berghei ANKA Liver
No difference
RMgm-156 Imported from RMgmDB

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