Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Refractory
USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible
PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. berghei ANKA Asexual
No difference
PlasmoGEM (Barseq) PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Immunofluorescence of plasmodial TPK. Immunofluorescence analyses were performed in 3D7 parasites expressing a cytosolic GFP reporter protein (Struck et al., 2005). The image shows single infection of an erythrocyte by P. falciparum (trophozoite stage), which was fixed onto a glass microscope slide and incubated with the DNA-specific dye DAPI (DAPI), a monoclonal anti-GFP antibody (Anti-GFP) and anti-PfTPK antibodies (Anti-PfTPK), respectively. The phase contrast image (Phase) was analysed in the merge image (Merge) revealing co-localisation of the GFP reporter and TPK protein – TPK is localized in the parasite’s cytoplasm.Eschbach ML, Müller IB, Gilberger TW, Walter RD, Wrenger C. The human malaria parasite Plasmodium falciparum expresses an atypical N-terminally extended pyrophosphokinase with specificity for thiamine. Biol Chem. 2006 387:1583-91. Copyright Walter de Gruyter GmbH 2009.

See original on MMP

More information